Yes....use what we call the three-three-three setting.
turn all three knobs to three o'clock. Minor adjustments from there.
If this is your first boutique..there are several things to keep in mind:
1) The Route 66 is not a high gain amp. It is VERY sensitive to input. Input from your pickups, effects, fingers, pick...etc. It is a very dynamic amp.
2) If you are accustomed to a high gain amp, the 66 will be very different. Do not be discouraged. You will come to love it. Some players often hide behind distortion/gain. Unfortunately, you will not be able to do that with the 66. It will show every mistake. Initially you may not like that, but as time goes on, you'll be able to use the incredible articulation as an additional tool for expression.
3) This amp has an ef86 preamp tube. This tube takes pedals differently. You may have to adjust settings on your pedals.
4) The tone controls are BEFORE the ef86, so turning up the bass and treble knobs will also add gain. Usually, up to a certain point it just adds bass or treble...but once past a certain point...it adds gain too.
5) Do NOT be afraid to turn the tone controls BELOW noon. As guitar players, we're accustomed to always having the tone controls of our amps higher than noon. Experiment with settings below 12:00. Try turning them with your eyes closed.
6) if you haven't purchased an attenuator like the brakelite...you might want to do that.
The Route 66 (and it's sister amps, KT-45 and the Z-28...they all share the same preamp) is a very different amp from the traditional Fender/Marshall. The controls act different, the amp feels different, the dynamics are different...it's a very different amp.
And welcome to the forum.